Chuang-Rung Chang

Examining yeast mitochondrial network morphology by soft X-ray tomography

Chuang-Rung Chang (張壯榮)

Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan

Department of Medical Science, National Tsing Hua, Hsinchu, Taiwan

Abstract:

Mitochondria are multi-function organelles in the cell. Most of cellular energy demand are supplied by respiration complexes located in the inner membrane of mitochondria through oxidative phosphorylation. Mitochondria also involve in the regulation of apoptosis, lipid metabolism and calcium homeostasis. Dynamic fusion, fission and transport are unique features of mitochondria. The balance of these processes cause formation of dynamic mitochondrial network. Maintaining mitochondria dynamics is critical for mitochondrial function. Yeast cells are primary eukaryotic cells that contain mitochondria. Most of the regulation mechanisms of mitochondria dynamics are preserved between yeast and mammalian models. Our lab had demonstrated mitochondria dynamics is linked to senescence and desiccation tolerance by yeast model. To further characterize the effects of environmental stress on mitochondria dynamics, we applied soft-X-ray tomography to examine mitochondrial network under stress conditions. We have gained preliminary images of yeast mitochondria. The results indicate that cell survival under environmental stress form long-term culture requires cell to be able to alter the balance of mitochondria dynamics.

Keywords – Mitochondria, Dynamics, Yeast.

References:

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