Abstract:
Macro domains, an ancient and diverse protein family, are key players in the recognition, interpretation, and turnover of ADP-ribose signaling in the form of a derived metabolite or as a modification covalently bound to proteins. Due to macro domains sharing similar fold but regulating a wide variety of cellular processes, we attempted to extensively study this protein family and zoom in on molecular details by using structural biology, along with biochemical and biophysical approach. Intriguingly, we found several previously unperceived events, including the NAD metabolites binding of viral macro domains, the novel yeast 3”-O-Acetyl-ADP-ribose deacetylase, and the existence of a poly-ADP-ribosylation system in bacteria. The findings not only expand the structural and functional diversification of Macro domains in microorganism but support their novel biological roles and emerging potential as therapeutic targets.
Keywords – macro domain; ADP-ribose; coronavirus; poly-ADP-ribosylation; OAADPR
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